|Cross-section of the engineered tissue showing cartilage (red) filling |
in between and around the bundles of polymer fibers (white).
Credit: Courtesy of Charles Gersbach and Farshid Guilak
Performing tissue repair with stem cells typically requires applying copious amounts of growth factor proteins—a task that is very expensive and becomes challenging once the developing material is implanted within a body.
However, in the new study the researchers found a way around this limitation by genetically altering the stem cells to make the necessary growth factors all on their own.
They incorporated viruses used to deliver gene therapy to the stem cells into a synthetic material that serves as a template for tissue growth. The resulting material is like a computer; the scaffold provides the hardware and the virus provides the software that programs the stem cells to produce the desired tissue.
The study appears online in this week's Proceedings of the National Academy of Sciences (PNAS).
Farshid Guilak, director of orthopaedic research at Duke University Medical Center, has spent the last years developing biodegradable synthetic scaffolds that mimic the mechanical properties of cartilage. One challenge he and all biomedical researchers face is getting stem cells to form cartilage within and around the scaffolds, especially after it is implanted into a living being.
The traditional approach has been to introduce growth factor proteins, which signal the stem cells to differentiate into cartilage. Once the process is under way, the growing cartilage can be implanted where needed.
"But a major limitation in engineering tissue replacements has been the difficulty in delivering growth factors to the stem cells once they are implanted in the body. There’s a limited amount of growth factor that you can put into the scaffolding, and once it’s released, it’s all gone. We need a method for long-term delivery of growth factors, and that’s where the gene therapy comes in." said Guilak, who is also a professor in Duke’s Department of Biomedical Engineering.
For ideas on how to solve this problem, Guilak turned to his colleague Charles Gersbach, an assistant professor of biomedical engineering and an expert in gene therapy. Gersbach proposed introducing new genes into the stem cells so that they produce the necessary growth factors themselves.
But the conventional methods for gene therapy are complex and difficult to translate into a strategy that would be feasible as a commercial product.
|A microscopic view using electron microscopy of human stem cells and viral gene carriers adhering to the fibers of a polymer scaffold. Photo Credit: Charles Gersbach and Farshid Guilak, Duke University|
This type of gene therapy generally requires gathering stem cells, modifying them with a virus that transfers the new genes, culturing the resulting genetically altered stem cells until they reach a critical mass, applying them to the synthetic cartilage scaffolding and, finally, implanting it into the body.
"There are a few challenges with that process, one of them being that there are way too many extra steps. So we turned to a technique I had previously developed that affixes the viruses that deliver the new genes onto a material’s surface." said Gersbach.
The new study uses Gersbach’s technique—dubbed biomaterial-mediated gene delivery—to induce the stem cells placed on Guilak’s synthetic cartilage scaffolding to produce growth factor proteins. The results show that the technique works and that the resulting composite material is at least as good biochemically and biomechanically as if the growth factors were introduced in the laboratory.
"We want the new cartilage to form in and around the synthetic scaffold at a rate that can match or exceed the scaffold’s degradation. So while the stem cells are making new tissue (in the body), the scaffold can withstand the load of the joint. In the ideal case, one would eventually end up with a viable cartilage tissue substitute replacing the synthetic material." said Jonathan Brunger, a graduate student who has spent time in both Guilak’s and Gersbach’s laboratories developing and testing the new technique.
While this study focuses on cartilage regeneration, Guilak and Gersbach say that the technique could be applied to many kinds of tissues, especially orthopaedic tissues such as tendons, ligaments and bones. And because the platform comes ready to use with any stem cell, it presents an important step toward commercialization.
"One of the advantages of our method is getting rid of the growth factor delivery, which is expensive and unstable, and replacing it with scaffolding functionalized with the viral gene carrier. The virus-laden scaffolding could be mass-produced and just sitting in a clinic ready to go. We hope this gets us one step closer to a translatable product." said Gersbach.
The research was funded part by the Nancy Taylor Foundation for Chronic Diseases, the Arthritis Foundation, the AO Foundation, the National Science Foundation and the National Institutes of Health.
- Brunger, J.M., Huynh, N.P.T., Guenther, C.M., Perez-Pinera, P., Moutos, F.T., Sanchez-Adams, J., Gersbach C.A., and Guilak F. (2014). Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. PNAS Plus DOI: 10.1073/pnas.1321744111