Tuesday, 3 September 2013

Researchers generate recombinant antibody that can isolate mesenchymal stem cells from umbilical cord blood

Mesenchymal Stem Cell
A new study published in BioResearch Open Access describes a recombinant antibody that can detect and isolate mesenchymal stem cells (MSCs), a type of adult stem cells with potential applications in tissue regeneration, blood stem cell transplantation, and treating several immune-related disorders.

The antibody works by recognising the i antigen (linear poly-N-acetyllactosamine) present on MSCs in umbilical cord blood. You can read the whole article by clicking the link provided on the references.

In their article, the authors explain how the i antigen can serve as a marker to detect and isolate MSCs in umbilical cord blood.

They describe the use of antibody phage display technology to produce a recombinant anti-i antibody that recognises i antigen on the surface of  umbilical cord blood-MSCs as well as on red blood cells. As of now, there are no commercially available antibodies that can do this, said the authors.

"The authors have used antibody phage display technology to generate an anti-i antibody. The advantage of this technique is that antibodies against poorly immunogenic molecules can be generated, as an immunization strategy is not required. The availability of an anti-i antibody has the potential to improve the isolation efficiency of MSCs from umbilical cord blood samples." said BioResearch Open Access Editor Jane Taylor, PhD, MRC Centre for Regenerative Medicine, University of Edinburgh, Scotland

Tia Hirvonen, Heli Suila, Sari Tiitinen, Suvi Natunen, Marja-Leena Laukkanen, Annika Kotovuori, Mirka Reinman, Tero Satomaa, Kaija Alfthan, Saara Laitinen, Kristiina Takkinen, Jarkko Räbinä, and Leena Valmu. BioResearch Open Access. -Not available-, ahe (2013). Production of a Recombinant Antibody Specific for i Blood Group Antigen, a Mesenchymal Stem Cell Marker BioResearch Open Access DOI: 10.1089/biores.2013.0026

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